Mutation type |
Mutation code |
Description |
Substitution |
G31C |
changing “G31” in original sequence to “C”. |
|
AT30G |
changing “A30T31” to “G”.
|
|
TGTGGGGTTAATCTGCCGCCGGAATGGGTAACCCA
GGAAATTTTT32GATGCTGCTGATAAATGCCCGATG
ACGCCTCCACATGTAAAACGT |
changing the “T32-T76” sequence to “GATGCTGCTGATAAATGCCCGA TGACGCCTCCACATGTAAAACGT”. |
Insertion |
A32insT |
inserting “T” behind “A32” |
|
C31insCATCATCATCACAGCAGCGGCCATATCGAAG
GTCGT |
inserting“CATCATCATCACAGCAGCGGCCATAT
CGAAGGTCGT” behind “C31”. |
Deletion |
GT30del |
deleting “G30” and “T31”. |
|
ATTTCCAATACAAGAGTTCCCTGAGGGATGGAGAG
GGGAATGTGGGCTATACGCGGTCGGATTCACAAA
ACGTGGGATTAGTGGAGCATCAATGGATGCAAAG
AGAATAGCTGAAGACATACACAAGTGTTGGAAA29del |
deleting “A29” to “A164”. |
[Monovalent cations]
mM
[Mg++ Conc]
mM
[dNTP Conc]
mM
[DMSO Conc]
mM
